TABLE 1.
Oligonucleotides used for PCR in this study
| Oligonucleotide | Sequence (5′–3′) | Gene | Usage |
|---|---|---|---|
| XBP1dir | CTGGAACAGCAAGTGGTAGA | XBP-1 | Detection of XBP1u, XBP1s, and XBP1es |
| XBP1rev | CTGGGTCCTTCTGGGTAG | XBP-1 | |
| xbpmacEX2-5dir | TAGCAGCTCAGACTGCCAGA | XBP-1 | Alternative splicing exons 2 to 5 of XBP1 |
| xbpmacEX2-5rev | ACTGGGTCCAAGTTGTCCAG | XBP-1 | |
| xbpmacEX1-3dir | GGGGCCCCTAAAGTACTGCT | XBP-1 | Alternative splicing exons 1 to 3 of XBP1 |
| xbpmacEX1-3rev | TCTTTAGCAACCAGGGCATC | XBP-1 | |
| snoU6up | CCAATGATGAGTTGCCATGC | snoU6 | Control of cell fractionation |
| snoU6lo | GCCCCTCAGATCTTCATGTG | snoU6 | |
| H_SS_475F22_qPCR | CGGAAGCCAAGTCTGATATCCT | XBP-1 | Quantitative detection of XBP1es (junction exons 3 to 5) |
| H_SS_625R21_qPCR | CTGATGACGTCCCCACTGACA | XBP-1 | Quantitative detection of XBP1es (exon 5) |
| GAPDH dir | GGAGCCAAAAGGGTCATCATCTC | GAPDH | Control for PCR loading and cell fractionation |
| GAPDH rev | TCCACAGTCTTCTGGGTGGCAG | GAPDH | |
| NPR2esmacup | CATGTTTGGTGTTTCCAGCTTCC | NPR2FL | Alternative splicing in NPR2FL |
| NPR2esmaclo | CGGGTTAGCTCAATGCGCTT | NPR2FL | |
| GOLGAesmacup | TCAAGAGAACCTACTTAAGCGTTGTAAGG | GOLGA4 | Alternative splicing in GOLGA4 |
| GOLGAesmaclo | TGAGCAATTTCTTCTTCTTTCATTTCC | GOLGA4 | |
| BAZesmacup | TGCTCTGATGGTTTTGGAGTTCC | BAZ1A | Alternative splicing in BAZ1A |
| BAZesmaclo | CGTTTTTGATATCTATACTTTGC | BAZ1A | |
| PAMesmacup | TGTCCCAGTGCCCGGG | PAM | Alternative splicing in PAM |
| PAMesmaclo | GGTGAAATCCACAGCTGACTTGG | PAM | |
| NCOAesmacup | AGGCAACACGACGAAACAGCCATACC | NCOA1 | Alternative splicing in NCOA1 |
| NCOAesmaclo | TCTGGCATAAGATGGCTCTCTGCCC | NCOA1 |