FIG 5.

HIV infection did not influence HAd5-specific CD4 T-cell frequencies, cytokine profiles, or PD-1 expression. (A) Percentages of HAd5-specific CD4 T cells producing IFN-γ, TNF-α, IL-2, or IL-4/5/13 of individuals with LTBI (n = 9) or HIV/LTBI (n = 10). (B) Proportion of HAd5-specific CD4 T-cell populations producing IFN-γ, IL-4/5/13, TNF-α, and/or IL-2 of individuals with LTBI (n = 9) or HIV/LTBI (n = 10). All the possible combinations of the responses are shown on the x axis, and the percentages of the functionally distinct cell populations within the HAd5-specific CD4 T-cell populations are shown on the y axis. Responses are grouped and color-coded on the basis of the number of functions. The pie chart summarizes the data, and each slice corresponds to the fraction of HAd5-specific CD4 T cell response with a given number of functions within the responding CD4 T-cell population. Bars correspond to the fractions of different functionally distinct CD4 T-cell populations within the total CD4 T cells. (C and D) Percentages of HAd5-specific CD4 T cells isolated from individuals with LTBI (n = 9) or HIV/LTBI (n = 10) expressing PD-1 (C) and/or CCR7 (D). Statistical significance (*; P < 0.05) was calculated using the Mann-Whitney test (A, C, and D). Statistical analyses of the global cytokine profiles (pie charts [B]) were performed by partial permutation tests using the SPICE software as described previously (54).