FIG 7.

No signs of anamnestic expansion of vaccine-induced T-cell responses or de novo induction of SIV-specific T-cell responses in r10091 after the 6th SIV exposure. (A) SIV-specific CD8⁺ T-cell responses at week 2.4 after challenge 6. The antigen stimuli for this assay consisted of pools of SIV peptides corresponding to vaccine-encoded SIV proteins (Vif, Nef, and Rev-Tat) or antigens that were not included in the vaccine (Gag, gp120, gp41, and Vpr-Vpx). The results are shown as contour plots displaying the percentages of CD8⁺ T cells producing IFN-γ and/or TNF-α in the absence of stimulation (no stimulus) or in response to the aforementioned SIV peptide pools. (B) Baseline (week of the 1st SIV challenge) levels of vaccine-induced CD8⁺ T-cell responses against Vif, Nef, and Rev-Tat are shown as a reference. In all panels, the cells were gated on live CD14⁻ CD16⁻ CD20⁻ CD3⁺ CD4⁻ CD8⁺ lymphocytes.