FIG 1.

CRISPR/Cas9-mediated genome disruption of bovine and porcine DNAJC14. (A) Scheme of the plentiCRISPRv2-DNAJC14 vector. (B) Illustration of the Bos taurus DNAJC14 gene with intron and exon composition (upper) and of the Sus scrofa DNAJC14 gene. The sgRNA sequence (5′-ATGATGTGCAGCCGATGCCA-3′) and the protospacer adjacent motif (PAM) targeting the Jiv90 domain of the bovine DNAJC14 gene in exon 4 is indicated below the gene composition as a black bar. (C) Scheme of DNAJC14-KO cell generation.