FIG 7.

Assessment of the capacity of the BVDV NS3-3′ replicon to support assembly of a minimal replicase and viral RNA replication in DNAJC14-KO and DNAJC14-KO GST-Jiv90 rescue cells by luciferase assay. (A) Schematic representation of the BVDV Bici-388 RLuc-NS3-3′ replicon. (B) In vitro-transcribed BVDV Bici-388 RLuc-NS3-3′/WT and BVDV Bici-388 RLuc-NS3-3′/GAA replicon RNA (2 μg) was electroporated into naive MDBK and MDBK DNAJC14-KO cells as well as MDBK DNAJC14-KO GST-Jiv90(WT) and MDBK DNAJC14-KO GST-Jiv90(W39A) rescue cells, and cells were collected at 2, 24, and 48 hpe to determine luciferase activity. Mean values from three independent experiments are shown. Error bars indicate standard deviations. Mock, no RNA electroporated; WT, wild type; GAA, polymerase-inactive mutant; RLUs, relative light units; RLuc, Renilla luciferase.