FIG 2.

Comparison between human and macaque NTCPs. Schematic representation of the primary amino acid and nucleotide sequence of NTCP. The 13 amino acid positions at which human (NM_003049.3) and cynomolgus macaque (NM_001283323.1) sequences differ are indicated by the numbers above the box showing the NTCP protein and the vertical lines (in red and blue). Among these 13 positions, five residues (indicated in red) were inferred to be under positive selection in the 20 mammalian sequences evaluated in this study. The amino acid sequences at residues 142 and 157 to 165 are compared between human and cynomolgus macaques, and their corresponding nucleotide differences are also highlighted in gray, as shown under the box. The indicated numbers correspond to the amino acid positions in human NTCP.