FIG 2.

Products generated by GtLPMO9B. (A) HPAEC-PAD chromatogram showing soluble native and oxidized cello-oligosaccharides released from PASC. The peaks were annotated based on Isaksen et al. (46); double-oxidized oligomers elute at 40 to 43 min. (B) HPAEC-PAD chromatogram showing soluble native and oxidized xyloglucan oligomers released from tamarind xyloglucan (TXG). Reaction mixtures contained 0.2% (wt/vol) PASC or TXG, 1 μM GtLPMO9B, and 1 mM AscA (solid line) or no AscA (dashed line) and were incubated in 50 mM bis-Tris-HCl buffer (pH 6.5) for 24 h at 45°C.