FIG 3.

Impairment of the ggaA gene postponed the formation of cellular biofilms and decreased the root colonization capability of the B. velezensis SQR9 strain. (A) Microtiter plate assay of biofilm formation by the wild-type and mutant strains. (B) OD₆₀₀ of solubilized crystal violet from the microtiter plate assay over time for the wild-type and mutant strains. Error bars indicate the standard deviations based on three different replicated experimental values. Different letters above the bars indicate significant differences (P < 0.05), and the subscript numbers differentiate the different time points. Mutant designations Δ2739, Δ9906, and Δ3020 represent mutants with deletions of the gene with the last four numbers of the GI accession number of each gene in the SQR9 genome in the NCBI database (accession no. CP006890), i.e., V529_33780 (https://www.ncbi.nlm.nih.gov/protein/631802739), V529_05450 (https://www.ncbi.nlm.nih.gov/protein/631799906), and V529_36590 (https://www.ncbi.nlm.nih.gov/protein/631803020), respectively.