FIG 5.

(A) Involvement of UDP-glucose in synthesis of WTAs and lipoteichoic acid. (B) Transcriptional levels of galE, ypfP, tagE, and gtaB in the ggaA mutant strain relative to those in wild-type strain SQR9. The experiments were carried out with pellicle obtained from microtiter plates when cells were grown in MSgg medium for 24 h. The B. velezensis SQR9 recA gene was used as an internal reference gene. RQ represents the relative expression level (relative quantification) compared to the wild-type strain. Bars represent standard deviations of data from three biological replicates.