TABLE 2.
Primers used in this study
| Primera | Oligonucleotide sequence (5′–3′) |
|---|---|
| PggaALF-F | TTTTGTATCAAAGCCTATCGT |
| PggaALF-R | GCTAGACAATAAGAGGAGAATTTAAAGTGATGATTGTGATTGGATATG |
| PggaADR-F | CGTATAGCATACATTATACGAACGGCGGTTCATCGCCATCTTCTC |
| PggaADR-R | TAGCGGTAGTCGTTCCTGTT |
| PggaARF-F | CATATCCAATCACAATCATCACTTTAAATTCTCCTCTTATTGTCTAGC |
| PggaARF-R | CGCAGTTTAAACAGGCGTAATAAAGTTCAACCTGCAAAAACAAAAAAAGG |
| PggaAPS-F | CCTTTTTTTGTTTTTGCAGGTTGAACTTTATTACGCCTGTTTAAACTGCG |
| PggaAPS-R | GAGAAGATGGCGATGAACCGCCGTTCGTATAATGTATGCTATACG |
| PgtaBLF-F | TCAAACTCTTCAACGACTCT |
| PgtaBLF-R | GGATTCGGCAGCTTTTTTGAATAACACCATCCTTGT |
| PgtaBDR-F | CGTATAGCATACATTATACGAACGGTGCTTCCAATCGTTGATAAG |
| PgtaBDR-R | GGCGTCCGTTAATTGAATT |
| PgtaBRF-F | ACAAGGATGGTGTTATTCAAAAAAGCTGCCGAATCC |
| PgtaBRF-R | CGCAGTTTAAACAGGCGTAATAAGACGGATGTTCAGCTTAATG |
| PgtaBPS-F | CATTAAGCTGAACATCCGTCTTATTACGCCTGTTTAAACTGCG |
| PgtaBPS-R | CTTATCAACGATTGGAAGCACCGTTCGTATAATGTATGCTATACG |
| PnucBLF-F | ATCAGGGTCTGCTTTTGTTT |
| PnucBLF-R | AAACTCCGGCGAACCGGAGAAGCTATCACATCCTCCTTAAGAAGT |
| PnucBDR-F | CGTATAGCATACATTATACGAACGGTTGGTGATCTCTCTTCAGTTCG |
| PnucBDR-R | CTCTCGTGCCGTCGGAATA |
| PnucBRF-F | ACTTCTTAAGGAGGATGTGATAGCTTCTCCGGTTCGCCGGAGTTT |
| PnucBRF-R | CGCAGTTTAAACAGGCGTAATAAGTTACCGCAGGGGGCCAGAATGATT |
| PnucBPS-F | AATCATTCTGGCCCCCTGCGGTAACTTATTACGCCTGTTTAAACTGCG |
| PnucBPS-R | CGAACTGAAGAGAGATCACCAACCGTTCGTATAATGTATGCTATACG |
| PyngKLF-F | TCCGCCCGCCGTCTAATGTATATAAAAATT |
| PyngKLF-R | AAGCAGAGAGGGGGAAAAACTAGCATTATTAATAAAACTGACAGTTGCGGCGC |
| PyngKDR-F | CGTATAGCATACATTATACGAACGGGAAGCCTGTCAACGGAAGTAACGATAT |
| PyngKDR-R | GGGTGCGGAACTACGATGATTTATACG |
| PyngKRF-F | GCGCCGCAACTGTCAGTTTTATTAATAATGCTAGTTTTTCCCCCTCTCTGCTT |
| PyngKRF-R | CGCAGTTTAAACAGGCGTAATAAGATCATTCTTGCCAGCTACC |
| PyngKPS-F | GGTAGCTGGCAAGAATGATCTTATTACGCCTGTTTAAACTGCG |
| PyngKPS-R | ATATCGTTACTTCCGTTGACAGGCTTCCCGTTCGTATAATGTATGCTATACG |
| PyyaHLF-F | GCAGGCTACTTAGGGCT |
| PyyaHLF-R | AGAAAAAAAGGGAGGCGGACATTAACAGTGCGGGGGAAC |
| PyyaHDR-F | CGTATAGCATACATTATACGAACGGCCATCCTCTATCACGCTCTCAT |
| PyyaHDR-R | GCAGCGATATGGGTGAAAGATT |
| PyyaHRF-F | GTTCCCCCGCACTGTTAATGTCCGCCTCCCTTTTTTTCT |
| PyyaHRF-R | CGCAGTTTAAACAGGCGTAATAATTCTGGTCGGATGCCATGATAT |
| PyyaHPS-F | ATATCATGGCATCCGACCAGAATTATTACGCCTGTTTAAACTGCG |
| PyyaHPS-R | ATGAGAGCGTGATAGAGGATGGCCGTTCGTATAATGTATGCTATACG |
| P2739LF-F | TTACGACGAAAAAGTATCCGAC |
| P2739LF-R | AGGAACATGGTGACTGTCCTTGATATGACATATCGAACACCG |
| P2739DR-F | CGTATAGCATACATTATACGAACGGACCATTCCAGCGTTCTCCAT |
| P2739DR-R | CCGACAGATCATGCCGATTATG |
| P2739RF-F | CGGTGTTCGATATGTCATATCAAGGACAGTCACCATGTTCCT |
| P2739RF-R | CGCAGTTTAAACAGGCGTAATAATTGACCACTCCGTCGTATTC |
| P2739PS-F | GAATACGACGGAGTGGTCAATTATTACGCCTGTTTAAACTGCG |
| P2739PS-R | ATGGAGAACGCTGGAATGGTCCGTTCGTATAATGTATGCTATACG |
| P9906LF-F | AGATATCGATCCTGCGATTT |
| P9906LF-R | CGGCTTTTGCGGTCTTTTTATTCAAGAACTCCTTTGCCTT |
| P9906DR-F | CGTATAGCATACATTATACGAACGGAACAGCCAATCTTCAGGAG |
| P9906DR-R | CGTCAGTCGGTGTATAGTC |
| P9906RF-F | AAGGCAAAGGAGTTCTTGAATAAAAAGACCGCAAAAGCCG |
| P9906RF-R | CGCAGTTTAAACAGGCGTAATAACTACTTTGCGCCTTTTTTGG |
| P9906PS-F | CCAAAAAAGGCGCAAAGTAGTTATTACGCCTGTTTAAACTGCG |
| P9906PS-R | CTCCTGAAGATTGGCTGTTCCGTTCGTATAATGTATGCTATACG |
| P3020LF-F | CGTATAACGTTTCTGTCGCATTAC |
| P3020LF-R | CTATTGAAAAGGGGAAAAAGCATAAGAAAAGGTGCCGTTTATCG |
| P3020DR-F | CGTATAGCATACATTATACGAACGGATGAACTCGCTGCCATTGTAA |
| P3020DR-R | GCTCGGATTGATGCTGACTG |
| P3020RF-F | CGATAAACGGCACCTTTTCTTATGCTTTTTCCCCTTTTCAATAG |
| P3020RF-R | CGCAGTTTAAACAGGCGTAATAAGACTTTGAATGGAAACGAAACG |
| P3020PS-F | CGTTTCGTTTCCATTCAAAGTCTTATTACGCCTGTTTAAACTGCG |
| P3020PS-R | TTACAATGGCAGCGAGTTCATCCGTTCGTATAATGTATGCTATACG |
| ggaA-qF | GTCCAAGCCGTCCGTAGAAG |
| ggaA-qR | ACAGGAACGACTACCGCTACCT |
| gtaB-qF | CGTTGATAAGCCGACAATTCAG |
| gtaB-qR | CATTGTCGAAATGGTCTTCTATTG |
| ggaALF-F | GAGAAGGCGTCGTAAAC |
| ggaALF-R | TAACGGCAGTAAAGAGGT |
| ggaACm-F | ATTCAAAACCTCTTTACTGCCGTTAGCATAAAGTGTAAAGCCTGGGG |
| ggaACm-R | CCTTTCGCTGAAAGTAACAAAATGAAATGTGGAATTGGGAACGGAAA |
| ggaACG-F | TCATTTTGTTACTTTCAGCG |
| ggaACG-R | ATACAAACCATTCTGCACATAATCAGAACACAGAAAGCTGATTCT |
| ggaARF-F | TGATTATGTGCAGAATGGT |
| ggaARF-R | TCTCGATAATATGGTAGGC |
| gtaBLF-F | GAGAAGGCGTCGTAAAC |
| gtaBLF-R | TAACGGCAGTAAAGAGGT |
| gtaBCm-F | ATTCAAAACCTCTTTACTGCCGTTAGCATAAAGTGTAAAGCCTGGGG |
| gtaBCm-R | AAAACCAGATATCGTTTTGGTCCACAATGTGGAATTGGGAACGGAAA |
| gtaBCG-F | GTGGACCAAAACGATATCT |
| gtaBCG-R | ATACAAACCATTCTGCACATAATCACTATTCGTCAGCTTCTTCTT |
| gtaBRF-F | TGATTATGTGCAGAATGGT |
| gtaBRF-R | TCTCGATAATATGGTAGGC |
a
Designations 2739, 9906, and 3020 refer to the genes with the last four numbers of the GI accession number in the SQR9 genome in the NCBI database (accession no. CP006890), i.e., V529_33780 (https://www.ncbi.nlm.nih.gov/protein/631802739), V529_05450 (https://www.ncbi.nlm.nih.gov/protein/631799906), and V529_36590 (https://www.ncbi.nlm.nih.gov/protein/631803020), respectively.