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ΔemrA decreases growth of CCCP-evolved strains in the presence of 150 µM CCCP. The growth of C-A1-1, C-A3-1, C-G5-1, C-B11-1, and C-D11-1 (light gray) with 150 µM CCCP in LBK (pH 6.5) with 100 mM Na-PIPES (A) and LBK (pH 8.0) with 100 mM TAPS (B) is compared to the growth of C-A1-1 ΔemrA::kanR, C-A3-1 ΔemrA::kanR, C-G5-1 ΔemrA::kanR, C-B11-1 ΔemrA::kanR, and C-D11-1 ΔemrA::kanR (dark gray). *, significant Tukey’s test P value of <0.05.
