FIG 6.

Growth of CCCP-evolved strains compared to the ancestor in LBK with 100 mM MOPS (pH 7.0) and 4 µg/ml chloramphenicol (A), 1 µg/ml tetracycline (B), 1 µg/ml ampicillin (C), or 6 µg/ml nalidixic acid (D). Curves shown are representative of the 8 replicates for each strain. Endpoint OD₆₀₀ data at 16 h as a measure of culture growth are shown. For chloramphenicol, growth of strains C-A1-1, C-D11-1, and C-F9-1 compared to W3110 gave a significant Tukey’s test P value of <0.05. For tetracycline, all strains except C-B11-1 and C-G5-1 had growth differing significantly from that of W3110 (P < 0.05). For ampicillin and nalidixic acid, growth of all evolved isolates compared to that of W3110 gave a Tukey’s test P value of <0.05.