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OD600 values for CCCP-evolved strains cultured with 100 mM MOPS, pH 7.0, over 22 h. (A) Strains evolved at low pH; W3110 differs significantly from all strains but H5-1 (P < 0.05; ANOVA with Tukey post hoc test, F = 15.925, P < 0.001; n = 8 for each strain). (B) Strains evolved at high pH; W3110 differs significantly from A9-1 and B11-1 (P < 0.05; ANOVA with Tukey post hoc test, F = 10.721, P < 0.001; n = 8 for each strain).
