FIG 1.

Effect of smu_833 deletion on protein expression. (A) Coomassie-stained SDS-PAGE gel comparing total protein profiles of the smu_833 mutant and its parent strain, UA159. Bracket indicates bands with decreased expression. (B) SDS-PAGE and Western blot of Gtf proteins in both the whole-cell lysate and cell supernatant from wild-type (WT), smu_833 mutant (−), and complementation (+) strains. A GtfB-specific antibody was used in the Western blot. (C) qRT-PCR analysis of gtfB, gtfC, and gtfD gene expression levels in WT, smu_833 mutant, and complement strains. Data presented here represent the mean values from four independent experiments. ***, P < 0.001; **, P < 0.01; NS, not significant compared with the wild type. Statistical analysis was done using Student’s t test.