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Effect of smu_833 deletion on biofilm pH. Wild-type, smu_833 mutant, and complemented bacterial strains were allowed to form a biofilm on polystyrene plates for 16 h. pHRodo red conjugated to dextran was added after the formation of the biofilm. An increase in red is indicative of a decrease in pH. SYTO9 green was used to stain bacterial cells. Images are representative of those from three independent experiments.
