TABLE 2.
Plasmids used in this study
| Plasmid | Description | Reference or source |
|---|---|---|
| pET15b | Ampr vector with a T7 promoter | Novagen |
| pET28a | Kanr vector with a T7 promoter | Novagen |
| pEGFP | Ampr egfp cassette vector | Clontech |
| pET28a-EGFP | EGFP gene cloned into NdeI-BamHI site of pET28a | 55 |
| pET15b-LysPBC2 | LysPBC2 gene cloned into NdeI-BamHI site of pET15b | This work |
| pET15b-LysPBC2_K129E | LysPBC2 (K129E) gene cloned into NdeI-BamHI site of pET15b | This work |
| pET15b-LysPBC2_Q132A | LysPBC2 (Q132A) gene cloned into NdeI-BamHI site of pET15b | This work |
| pET15b-LysPBC2_K137E | LysPBC2 (K137E) gene cloned into NdeI-BamHI site of pET15b | This work |
| pET15b-LysPBC2_R142E | LysPBC2 (R142E) gene cloned into NdeI-BamHI site of pET15b | This work |
| pET15b-LysPBC2_R148E | LysPBC2 (R148E) gene cloned into NdeI-BamHI site of pET15b | This work |
| pET28a-LysPBC2_EAD | EAD fragment (Met1-Ser165) of LysPBC2 cloned into NdeI-BamHI site of pET28a | This work |
| pET28a-EGFP-PBC2_CBD | CBD fragment (Gly163-Asp311) of LysPBC2 cloned into BamHI-HindIII site of pET28a-EGFP | This work |
| pET28a-EGFP-PBC2_SBD1 | SBD fragment (Ala108-Thr170) of LysPBC2 cloned into EcoRI-XhoI site of pET28a-EGFP | This work |
| pET28a-EGFP-PBC2_SBD-core | SBD fragment (Arg127-Gly147) of LysPBC2 cloned into EcoRI-XhoI site of pET28a-EGFP | This work |