Day 3 jejunum metabolomics: (a) PLS-DA plot comparing sham, 8 Gy, and 10–14 Gy TBI doses; R2=0.94, Q2=0.91. Each point represents a data set from an individual animal. The 95% confidence intervals are indicated by elliptical patterns per group. Data were sum normalized, log transformed, and mean centered. (b) Volcano plot highlighting metabolites that were differentially expressed when comparing sham to 8 Gy. (c) Volcano plot highlighting metabolites that were differentially expressed when comparing sham to 10–14 Gy. (d) Volcano plot highlighting metabolites that were differentially expressed when comparing 8 Gy to 10–14 Gy.