Fig. 1. Plasma metabolites changed at 3 days post-8.72 Gy TBI.

(a) PLS-DA plot: naïve (red) v TBI (green); n=10 per group; female and male are grouped together; R2=0.99, Q2=0.79. Each point represents a data set from an individual animal. The 95% confidence intervals are indicated by elliptical patterns per group. Data were sum normalized, log transformed, and mean centered. (b) Volcano Plot: naïve v TBI; n=10 per group; female and male are grouped together. FC=fold change, p=pvalue. Red dots are metabolites with p<0.001, blue dots are metabolites with p<0.01, and orange dots are metabolites with p<0.05. (c) Class distribution of significantly changed metabolites listed in Table 1 (p<0.05). (d) Cit as representative of metabolites that are significantly changed in female and male grouped cohort as well as in female and male single-sex cohorts. (e) SM_36-2 as rpresentative of metabolites that are significantly changed in female and male grouped cohort and in female single-sex cohort only. (f) LPC_20–3 as representative of metabolites that are significantly changed in female and male grouped cohort as well as in female and male single-sex cohorts but have significant basal differences between female and male. All plasma metabolites assayed from 3 month old C57BL/6 mice; n=10 for each female and male grouped cohort (Naïve, IR) where each n=10 cohort had n=5 each of female and male. Mean ± SD; *p<0.05. M=male, F=female, IR=3 d post-8.72 Gy, naïve = sham.