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. Author manuscript; available in PMC: 2020 Mar 1.
Published in final edited form as: Am J Transplant. 2018 Sep 17;19(3):646–661. doi: 10.1111/ajt.15083

FIGURE 3.

FIGURE 3

HY-mismatched skin grafts from TORC2DC−/− male (M) donors exhibit enhanced CD8+PD-1+ T cell infiltration compared with grafts from WT control (Ctrl) M donors. Cells were isolated from skin grafts on post-operative day (POD) 7 via collagenase digestion and analyzed following mAb staining by flow cytometry. T cells were gated on live (Zombie-) CD45.2+CD3+ cells. (A) Total numbers of CD4+Foxp3- T effector (Teff) cells, CD8+ cells and Teff:CD4+Foxp3+ (Treg) cell ratios within the grafts. (B) Representative histograms of CD8+PD-1+ T cells within the grafts. (C) Numbers of CD8+PD-1+ T cells within the grafts (left) and mean fluorescence intensity (MFI) of PD-1 on PD-1+ cells (right). n=4 mice per group; one-way ANOVA Tukey’s multiple comparisons test, *, p < 0.05; **, p<0.01, ***, p<0.001, ****, p<0.0001.