MHC-mismatched skin grafts from TORC2DC−/− donors exhibit enhanced Banff rejection scores compared with grafts from Ctrl donors. Wild-type BALB/c mice received full-thickness skin grafts from either B6 WT control (Ctrl B6) or TORC2DC−/− B6 donors. (A) Graft survival over time; n=3–6 mice per group; Log-rank test. (B) Representative gross morphology of skin grafts at post-operative day (POD) 5 and POD 7. (C) Banff rejection scores of skin grafts at POD 5; n=4 mice per group; one-way ANOVA Tukey’s multiple comparisons test; *, p < 0.05; **, p < 0.01. (D) Representative H&E staining of skin grafts at POD 5 showing (above) the epidermal-dermal junction (E-D) and (below) the deep dermal layer (DD). Arrowheads indicate (1) vacuolar damage, (2) pathological diskeratosis, (3) thrombosis, and (4) vasculitis.