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. Author manuscript; available in PMC: 2020 Mar 1.
Published in final edited form as: Am J Transplant. 2018 Sep 17;19(3):646–661. doi: 10.1111/ajt.15083

Figure 7.

Figure 7

MHC-mismatched skin grafts from TORC2DC−/− donors elicit enhanced proliferation of CD8+ T cells in draining lymph nodes (LN) and augmented IFNγ and IL-2 production in response to donor Ag stimulation. T cells were isolated from the axillary LN of TORC2−/− or WT control (Ctrl) skin graft recipients (BALB/c) on POD 5. Isolated T cells were labeled with the cell proliferation dye CFSE, and stimulated with B6 splenic DC for 3 days. (A) Proliferation of CD4+Foxp3- T cells measured by cellular CFSE content (above, percent dividing; below, division index). (B) Proliferation of CD8+ T cells as measured by cellular CFSE content (above, percent dividing; below, division index). (C) Representative cell proliferation profiles from each group. (D) Levels of IFNγ, IL-2, and GrB in the culture supernatants. n=4 mice per group; one-way ANOVA Tukey’s multiple comparisons test, *, p < 0.05; **, p<0.01, ***, p<0.001, ****, p<0.0001.