Fig. 7. Effects of andrographolide on proliferation of myosin-treated splenetic cells.

(A) Splenetic cells were collected from EAM rats, and treated with andrographolide in the presence and absence of SC79 for 48 h. Myosin-specific proliferative responses were measured by MTT colorimetric technique. (B1) typical p-Akt stripes; (B2) the stripes were quantitatively analyzed by image j. Results are presented as the mean ± S.D. N = 3. Con: control; EAM: experimental autoimmune myocarditis. ^##p < 0.01, compared with splenetic cells without treatment; ^*p < 0.05, ^**p < 0.01, compared with splenetic cells only treated with myosin (20 µg/ml); ^Δp < 0.05, compared with the cells treated with myosin and andrographolide (40 µM).