Fig. 2. Alteration of intracellular Ca²⁺ concentration in HUVECs induced by tunicamycin.

HUVECs were treated with 1 µg/ml tunicamycin for 24 h. Cells were incubated in a Ca²⁺-free buffer containing 2 µM thapsigargin for 8 min, and Ca²⁺ influx was induced during the Ca²⁺ loading period (left panel). Ca²⁺ influx was enhanced by tunicamycin (right panel). ^**p < 0.01 vs. control; n = 60 cells.