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. 2019 Feb 15;23(2):95–102. doi: 10.4196/kjpp.2019.23.2.95

Fig. 5. Effect of Orai1 siRNA on tunicamycin-induced eNOS and UPR markers expression.

Fig. 5

HUVECs were preincubated with 50, 100, or 200 MOI adenovirus-Orai1siRNA for 24 h; 1 µg/ml tunicamycin was added for another 24 h. (A) eNOS, UPR markers and Orai1 expression were assayed by western blotting. (B-E) Densitometric analysis of the protein levels of eNOS, BiP, Xbp1-s and Orai1 respectively, GAPDH was used as the internal control. *p < 0.05, **p < 0.01 vs. control; #p < 0.05, ##p < 0.01 vs. tunicamycin treatment only, n = 4.