. 2019 Feb 15;10:207. doi: 10.3389/fimmu.2019.00207

Table 4.

C. burnetii HLA class II epitope immunogenicity in tgHLA-DR3 mice.

Peptide ID	No. of mice responding^d	Mouse 1/3			Mouse 2/3			Mouse 3/3
		SFU^a	SI^b	p-value^c	SFU^a	SI^b	p-value^c	SFU^a	SI^b	p-value^c
p2	2	120	6.63	0.0003	141	8.28	< 0.0001	18	1.53	0.1384
p10	2	256	5.80	< 0.0001	82	4.51	0.0043	43	2.00	0.0496
p14	2	157	7.94	0.0006	371	20.21	0.0001	11	1.42	0.0149
p16	1	180	8.94	0.0007	27	2.05	0.0060	19	1.94	0.0164
p23	2	69	2.30	0.0038	21	1.89	0.0372	63	2.47	0.0172
p28	2	63	3.76	0.0013	54	3.79	0.0094	41	2.63	0.0227
p31	1	0	0.88	0.1143	14	1.72	0.0621	84	4.32	0.0007
p32	2	64	3.82	0.0053	70	4.62	0.0103	28	2.11	0.0609
p36	2	52	3.44	0.0468	55	3.86	0.0193	0	0.86	0.2215
p41	1	87	5.06	0.0052	30	2.55	0.0433	0	0.98	0.3566
p43	3	135	4.88	0.0001	65	3.04	0.0013	116	4.48	0.0001

Average number of spot forming units (SFU) per million splenocytes from triplicate wells; the average number of SFU/million in medium only control wells was subtracted. Negative values were assigned 0.

Stimulation index (SI) calculated as average number of SFUs in peptide stimulated wells over medium only control wells.

SFUs in peptide stimulated wells from immunized mice compared to mock-immunized mice by Student's t-test.

Mice are deemed responsive to a peptide if their SFU is >50 and if their SI is >2 and if their p-value is lower than 0.05.