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. 2019 Feb 15;10:141. doi: 10.3389/fpls.2019.00141

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Copyright © 2019 Fernandez-Göbel, Deanna, Muñoz, Robert, Asurmendi and Lascano.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Systemic redox changes are observed after root inoculation with B. japonicum. Leaves of non-inoculated (control, C) and inoculated (I) plants were sampled at different times after root inoculation and (A) total ascorbic acid, and (D) glutathione levels were measured. The reduced/total ratio of (B) ascorbic acid, and (E) glutathione was calculated. MDA content (C) was quantified. Data are means ± SE of three independent experiments. Asterisks indicate significant difference between control and inoculated treatments (p < 0.05, Tukey test).