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. 2019 Feb 15;10:141. doi: 10.3389/fpls.2019.00141

FIGURE 3.

FIGURE 3

NADPH oxidase complex modulates the systemic redox changes after root inoculation with B. japonicum. Leaves or roots of 12-days-old soybean plants were pretreated 30 min with 50 μM DPI (+DPI) or DMSO (–DPI), and then roots were inoculated with B. japonicum. After 30 min of root inoculation, leaves of non-inoculated (control, C) and inoculated (I) plants were sampled and incubated with (A) 3,3′-diaminobenzidine (DAB) for H2O2 quantification. The results are expressed relative to non-inoculated plants (assigned a value of 1). DAB precipitated in leaves was measured and transformed into optical density (OD) by the image processing software Optimas®. The results are the means of three independent experiments (four leaves per treatment). Different letters indicate significant differences between the treatments (p < 0.05, Tukey test). SOD (B), GR (C), CAT (D) and APX (E) activities were measured 30 min post inoculation in leaves of plants whose roots were pretreated with DPI (+DPI) or DMSO (–DPI). Data are means ± SE of three independent experiments. Asterisks indicate significant difference between control and inoculated treatments (p < 0.05, Tukey test).