Effects of (a) amount of t-butanol (mL) on EY of IOPS using a solid-liquid ratio 1:12, a mass fraction of (NH4)2SO4 30% (w/v), a time of 30 min, and a temperature of 30 °C at pH 8.0; (b) mass fraction of (NH4)2SO4 (%, w/v) on the EY of IOPS using a solid-liquid ratio of 1:12, t-butanol (10 mL), an extraction time of 30 min, and a temperature of 30 °C at pH 8.0; (c) temperature on the EY of IOPS using a solid-liquid ratio of 1:12, t-butanol (10 mL), a mass fraction of (NH4)2SO4 30% (w/v), and an extraction time of 30 min at pH 8.0; (d) pH on the yield of IOPS using a solid-liquid ratio of 1:12, t-butanol (10 mL), a mass fraction of (NH4)2SO4 of 30% (w/v), an extraction time of 30 min, and a temperature of 30 °C; (e) extraction time on the yield of IOPS using a solid-liquid ratio of 1:12, t-butanol (10 mL), a mass fraction of (NH4)2SO4 of 30% (w/v), and a temperature of 30 °C at pH 8.0.