Table 2.
Comparison of resolution of secondary alcohols between Magnetic CALB CLEAs and Novozym 435 a.
| Entry | Substrate | Catalyst | eep b(%) | ees b(%) | Conversion b (%) | E |
|---|---|---|---|---|---|---|
| 1 |
|
Magnetic CALB CLEAs | >99 | >99 | 50.0 | >1000 |
| Novozym 435 | 96 | >99 | 51.1 | 175 | ||
| Free CALB | >99 | 2 | 2.1 | 204 | ||
| Fe3O4 | >99 | 1 | 1.3 | 203 | ||
| APTES–Fe3O4 | >99 | 2 | 1.9 | 204 | ||
| 2 |
|
Magnetic CALB CLEAs | >99 | 94 | 48.4 | >1000 |
| Novozym 435 | 94 | 98 | 50.9 | 149 | ||
| 3 |
|
Magnetic CALB CLEAs | 95 | 43 | 31.0 | 56 |
| Novozym 435 | 35 | 74. | 68.1 | 4 | ||
| 4 |
|
Magnetic CALB CLEAs | 94 | 28 | 22.6 | 43 |
| Novozym 435 | 30 | 98 | 76.3 | 7 | ||
| 5 |
|
Magnetic CALB CLEAs | >99 | >99 | 50.0 | >1000 |
| Novozym 435 | 8 | >99 | 92.1 | 4 | ||
| 6 |
|
Magnetic CALB CLEAs | 95 | 7 | 7.1 | 39 |
| Novozym 435 | 97 | 63 | 39.2 | 128 | ||
| 7 |
|
Magnetic CALB CLEAs | / | 0 | 0 | / |
| Novozym 435 | >99 | <1 | 0.6 | 200 | ||
| 8 |
|
Magnetic CALB CLEAs | 92 | 22 | 19.4 | 31 |
| Novozym 435 | 91 | >99 | 52.1 | 109 | ||
| 9 |
|
Magnetic CALB CLEAs | >99 | 83 | 45.5 | 605 |
| Novozym 435 | 95 | >99 | 51.0 | 207 | ||
| 10 |
|
Magnetic CALB CLEAs | >99 | >99 | 50.0 | >1000 |
| Novozym 435 | 94 | >99 | 51.4 | 160 | ||
| 11 |
|
Magnetic CALB CLEAs | >99 | 78 | 44.0 | 472 |
| Novozym 435 | >99 | >99 | 50.0 | >1000 | ||
| 12 |
|
Magnetic CALB CLEAs | >99 | 25 | 20.1 | 254 |
| Novozym 435 | >99 | >99 | 50.0 | >1000 | ||
| 13 |
|
Magnetic CALB CLEAs | >99 | >99 | 50.0 | >1000 |
| Novozym 435 | >99 | >99 | 49.8 | >1000 | ||
| 14 |
|
Magnetic CALB CLEAs | >99 | >99 | 50.0 | >1000 |
| Novozym 435 | 97 | >99 | 50.7 | 374 | ||
| 15 |
|
Magnetic CALB CLEAs | >99 | >99 | 50.0 | >1000 |
| Novozym 435 | 95 | 2.8 | 2.8 | 45 |
a Reaction conditions: time 48 h, temperature 50 °C, 2 mL of n-hexane containing 0.2 mmol of alcohols and 0.4 mmol of vinyl acetate, and 25 mg Magnetic CALB CLEAs or 2 mg Novozym 435. b Determined by chiral HPLC analysis.