Figure 4.

Cloning and sequence analysis of pCsAlaDC from tea plants. (A) Prediction of the gene structure using online software Splign. The genome sequence of the gene was obtained from the “Shuchazao” genome. (B) Phylogenetic relationship of pCsAlaDC from the tea plants with amino-acid decarboxylases from other species. The sequences of amino-acid decarboxylases from seven plant species were obtained from the UniProt database. The alignment was conducted using the ClustalW method, and the phylogenetic analysis was performed using the neighbor-joining method with MEGA7 software; pCsAlaDC is denoted by a dot. SDC, serine decarboxylase; TYDC, tyrosine decarboxylase; DCE, glutamate decarboxylase; KDC, lysine decarboxylase; SPE or ADC, arginine decarboxylase. At, Arabidopsis thaliana; Os, Oryza sativa; Gm, Glycine max; Tc, Theobroma cacao; Ga, Gossypium arboreum; Vv, Vitis vinifera; Jc, Jatropha curcas.