Table 1.

Comparison of commonly used methods in quantitative proteomics.

Quantitation Methods	Advantages	Disadvantages	Metal Drugs Investigated by Each Method
ICAT	Procedure is easy.	Only two samples can be labeled, which is only applicable to proteins containing cysteine.	Cisplatin [43,44]
TMT	Quantification on multiple sets of protein samples.	Expensive	Cisplatin [45]
iTRAQ	Quantification on multiple sets of protein samples.	Expensive	Cisplatin [44]
SILAC	Applicable to cultured cell.	It cannot be applied to samples such as tissues and body fluids. Expensive, time-consuming, and complicated.	Cisplatin [46] Gold (III) porphyrins [35]
LFQP	Straightforward and cost-effective.	It requires more rigorous analytical measurements and statistical validation than isotope-coded measurements.	Cisplatin [47] [Pd(sac)(terpy)](sac) [48] Plecstatin [49] RAPTA agent [50]
2-DE MS	It resolves thousands of intact protein species in a single run.	Time-consuming and labor-intense.	Cisplatin [51] Auranofin [52] Auoxo6 [52] Gold (III) NHC complexes [53]