Table 2.
Dynamic and static differences of measurements with EcFOF1. The top part shows the dynamic differences in dwell times for the three catalytic dwells found in our single-molecule fluorescence experiments. For comparison, the bottom row shows the static distribution of the three different states in the cryo-EM measurement. (* FRET-levels do not apply to this method.)
| FRET-Level Method | M, Dwell Time 1 (ms) | L, Dwell Time 2 (ms) | H, Dwell Time 3 (ms) | Direction of Rotation |
|---|---|---|---|---|
| rotation assay* | 300.0 | 200.0 | 200.0 | ATP hydrolysis |
| smFRET ε56/b64 | 12.7 | 17.6 | 15.8 | ATP hydrolysis |
| smFRET ε56/b64 | 15.4 | 24.0 | 19.5 | ATP synthesis |
| Conformation | State 1 | State 2 | State 3 | |
| cryo-EM* | 47% | 30% | 24% | N.A. |