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. 2019 Jan 29;24(3):475. doi: 10.3390/molecules24030475

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© 2019 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Quantification of MH uptake by medaka embryos by liquid chromatography-mass spectrometry (MS). This figure is a typical MS profile for the analysis of 10 μM MH uptake following 24 h-incubation with embryos, and maintained at 26 ± 1 °C. The amount of MH disappearance from the conditioned media was measured at time 0 h and time 24 h. Selected ion monitoring (SIM) at m/z 281 shows signals for MH at time 0 h (A) and time 24 h (B). (C) The unknown molecule in MS scan mode. (D) The amount of disappearance of MH following 24 h incubation with embryo.