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Stages with regard to hours post fertilization of the medaka heart. This scheme outlines the embryological development of the heart during the first 144 h and subsequent formation of concentric chamber growth. The medaka heart begins beating and pumping around 44 (stage 24) to 50 (stage 25) hours post fertilization. Medaka embryos were treated with MH either from 0–6 days post fertilization or 2–6 dpf at 26 ± 1 °C.
