Figure A1.

Examples of images representing the medaka phenotype following MH treatment. Medaka embryos were treated with 10 μM MH or an untreated control batch (0.02% DMSO) for 0–6 dpf or 2–6 dpf following the same protocol as described in the method. (A) Control. No phenotypic abnormality was detected with the control for the two groups. This first image displays an example of a typical control obtained immediately for both treatment plans. (B) This is an example of a typical medaka treated with 10 μM MH for 0–6 dpf. Curved tail phenotypes were observed in many. (C) Edema phenotypes were observed in medaka treated with 10 μM MH for 2–6 dpf. Bent tail phenotypes were observed in few.