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Primers used in this study.
| Primer Name | Sequences (5′–3′) |
|---|---|
| SmsrtA-F | CGCGGATCCATGAAAAAAGAACGTCAATCTAGGA |
| SmsrtA-R | CCGCTCGAGTTAAAATGATATTTGATTATAGGACTGCC |
| R213A-SrtA-F | TTGTTCATGGCACATATAAGGGGGAA |
| R213A-SrtA-R | TTGTTGCAGCAGTCGCCC |
| L111A-SrtA-F | AAGGAGCAGATAATGTTGGCTTAAC |
| L111A-SrtA-R | TGAAGATTGGTAAATTGATTTTTAAGTCTGG |
| L116A-SrtA-F | TTGGCGCAACATATGGTG |
| L116A-SrtA-R | ATTTATCTAATCCTTTGAAGATTGGTAAATTG |
The underlined basic groups represent restriction endonuclease recognition sites or mutated codons.
