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The fluorescence emission spectra of ligand + HSA systems for (a) AML at λEX = 370 nm, (b) Q at λEX = 375 nm, and (c) Q at λEX = 450 nm. In all experiments, the ligand concentration was 7.5 μM and HSA concentrations were 0 (line 0), 1.87 (line 1), 3.75 (line 2), 7.5 (line 3), 15 (line 4), 20 (line 5), and 30 (line 6) μM. Conditions: T = 310.15 K, pH = 7.4.
