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Near-UV CD spectra of (a) AML + HSA, (b) Q + HSA, (c) (Q + HSA) + AML, and (d) (AML + HSA) + Q. Conditions: T = 310.15 K, pH = 7.4. The HSA concentration was 15 μM while the concentrations of AML or Q were 7.5 μM (red line), 15 μM (blue line), 30 μM (pink line), 45 μM (green line), and 60 μM (dark blue line).
