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. 2019 Feb 22;9:2573. doi: 10.1038/s41598-019-39119-z

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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smFISH localizes transcripts encoding the SLO channels K_Ca1.1, K_Na1.1, K_Na1.2 but not K_Ca5.1 to the spiral ganglion neurons (SGNs). Expression of K_Na1-encoding transcripts in the SGNs was examined using smFISH in excised preparations of the OC/SGN isolated from 6-week-old mice. (A) RNA molecules encoding for K_Ca1.1 (Kcnma1), K_Na1.1 (Kcnt1), K_Na1.2 (Kcnt2), K_Ca5.1 (Kcnu1) and no probe controls were detected as fluorescent puncta (green) in TUJ1-positive (red) SGN somas. For easier visualization of fluorescently labelled mRNA molecules, identical views are provided in which detected mRNA molecules are represented instead as spheres (green) and SGNs are shown in grey. (B) The mean number of RNA molecules detected per SGN was calculated as described in the Methods. Kcnma1 was most abundantly expressed, Kcnt1 and Kcnt2 were expressed at intermediate values, and Kcnu1 and no probe controls showed little to no expression. Data are plotted to show individual replicates (animals) and mean ± SEM. Values (mean ± SEM) are provided in the Results.