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. 2019 Feb 8;24(3):599. doi: 10.3390/molecules24030599

Table 1.

Purification yields of TfHex mutants and GalNAcase/GlcNAcase activity ratios.

Enzyme Protein Yield [mg] 1 GlcNAcase [U/mg] GalNAcase [U/mg] GalNAcase/GlcNAcase Ratio
WT TfHex 188 37.4 45.1 1.2
Tyr470Phe TfHex 308 0.3 0.3 1.0
Tyr470His TfHex 84 0.4 0.1 0.3
Tyr470Asn TfHex 225 1.6 0.2 0.1

Note: 1 Purification from 800 mL cultivation medium.