Skip to main content
. 2019 Feb 8;24(3):599. doi: 10.3390/molecules24030599

Table 2.

Kinetic parameters of pNP-GlcNAc and pNP-GalNAc hydrolysis by TfHex variants.

Enzyme Substrate Km [mM] kcat [s−1] kcat/Km [s−1 mM−1]
WT TfHex pNP-GlcNAc 0.11 ± 0.02 47 ± 1 434
pNP-GalNAc 0.69 ± 0.09 104 ± 5 150
Tyr470Phe TfHex pNP-GlcNAc 0.023 ± 0.008 0.27 ± 0.01 12
pNP-GalNAc 0.050 ± 0.005 0.166 ± 0.003 3.3
Tyr470His TfHex pNP-GlcNAc 0.053 ± 0.008 0.67 ± 0.02 13
pNP-GalNAc 0.35 ± 0.03 0.188 ± 0.004 0.53
Tyr470Asn TfHex pNP-GlcNAc 0.89 ± 0.13 11.6 ± 0.6 13
pNP-GalNAc 0.89 ± 0.09 0.35 ± 0.01 0.40