Figure 2.

Effects of MECC on the LDLR expression in HepG2 cells. HepG2 cells were cultured in LPDS medium and treated with vehicle (0.1% DMSO) or MECC (0.05 and 0.1 mg/mL) for 24 h. (a) The level of LDLR mRNA was measured by reverse transcription real-time PCR analysis. (b) The levels of LDLR and β-actin proteins were analyzed by Western blot analysis. The experiments were replicated three times, and a representative blot was shown. (c) The intensity of LDLR normalized to the β-actin protein is presented as the means ± SD of three independent experiments. * p < 0.05 and ** p < 0.01 represent significant differences compared to the vehicle-treated group.