Table 1.
Detection of SEPs using MS in Mycoplasma pneumoniae
| Type | N | Criteria | |||
|---|---|---|---|---|---|
| ≥ 1 UTP | ≥ 1 UTP; ≥ 1 NUTP | ≥ 2 UTP | |||
| SEPs | Annotated | 26 | 22 | 22 | 21 |
| Putative | 17,792 | 42 | 29 | 7 | |
| Decoy | 20,100 | 19 | 0 | 0 | |
The outcome of different results after MS searches using the decoy database (negative control) and translating all the possible ORFs in M. pneumoniae. When using the cutoff of at least 2 UTPs, the signal of every decoy protein was removed but the detection of putative SEPs consequently dropped, with one annotated SEP not being identified.