3-D response surface curves of the L-Met titer as a function of combinational effects of multiple variables. (a), glucose and yeast extract; (b), glucose and KH2PO4; (c), glucose and MgSO4.7H2O; (d), yeast extract and KH2PO4; (e), yeast extract and MgSO4.7H2O; (f), KH2PO4 and MgSO4.7H2O (f)