Figure 5.

Rayleigh interference optical sedimentation boundaries of 82 mg/mL chicken γS-crystallin in 50 mM Tris-HCl, 25 mM NaCl, 1 mM DTT, pH 7.5, sedimenting at 50 000 rpm in 1.75 mm path length centerpiece. For clarity only every 10^th data point of every 3^rd scan is shown (circles), and best-fit from the c_NI(s₀) model (lines). The ratio of rmsd of the residuals (lower panel) to loading signal is 0.0034.