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. 2018 Sep 6;30(2):461–475. doi: 10.1021/acs.bioconjchem.8b00524

Figure 5.

Figure 5

PPx-GALA polyplexes facilitate the delivery of the membrane-impermeable fluorescent molecule calcein into the cytosol of dendritic cells. Confocal microscopy (A) and flow cytometry analysis (n = 4) (B) of the cellular uptake of calcein in D1 cells after 3 h in the presence of serum at 37 °C after incubation of polymer, Px, and PPx-GALA in the presence of 150 μg/mL calcein (blue, nuclei; red, Cy5-luc mRNA; green, calcein). Scale bar: 10 μm. (C) The mean fluorescence intensity of calcein in D1 cell nuclei measured by ImageJ from confocal microscopy pictures. Data are the mean ± SD.