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. 2019 Jan 23;24(3):405. doi: 10.3390/molecules24030405

Figure 3.

Figure 3

Incorporation of the C2-D probe (orange) on the histidine imidazole ring was achieved by heating the tripeptide in D2O for three days at 50 °C in D2O at pH 8. To back exchange readily exchangeable hydrogens on nitrogen and oxygen (green), the sample was then re-dissolved in H2O, pH 2, and lyophilized.