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. 2019 Feb 22;9:2595. doi: 10.1038/s41598-019-39486-7

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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CeO₂ NP suppress intracellular ROS production induced by UVA radiation. HSFs were treated, washed, and stained as mentioned in the Materials and Methods section. ROS levels in HSF cells were measured by a flow cytometer (A) and an inverted fluorescence microscope (B) labelled with DCFH-DA for 30 min at 37 °C. Scale bar = 10 µm.