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. 2019 Feb 21;9:2516. doi: 10.1038/s41598-019-38676-7

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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The effect of afatinib on the cell survial of CTX-TNA2 cells and primary cultured astrocytes. CTX-TNA2 cells and primary cultured astrocytes were treated with afatinib (1–100 nM) for 24 h. (A,B) Cell viability of CTX-TNA2 cells and primary cultured astrocytes was determined using SRB assay. (C) Cell viability of primary cultured astrocytes was determined by LDH assay. Values are normalized to the no drug condition and are the mean ± S.E.M. (n = 3/group).