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. 2019 Feb 21;9:2524. doi: 10.1038/s41598-019-38851-w

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Live cell filopodial dynamics. (A) Selected frames of confocal time-lapse of a neurite of N1E-115 cell co-expressing mRFP-IRSp53 (not shown) and GFP-actin (intensity heat map look up table (LUT)). The arrows indicate the dynamic filopodia initially extends before retracting upon addition of Cytochalasin D (at 7 min). Images were recorded every 30 s for a total of 12 min. Scale bar = 5 µm. (B) Selected frames of a widefield fluorescence time-lapse showing N1E-115 cell co-expressing mRFP-I-BAR domain of IRSp53 (not shown) and GFP-actin (intensity heatmap LUT). Images were recorded every 30 s for a total of 12 min. The filopodia life time observed (n = 10) was >7 min and was unaffected by the addition Cytochalasin D (at 7 min). Scale bar = 5 µm.